Threshold Ventures

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Overview

Threshold Ventures is an early-stage venture capital firm founded in 2019 that invests primarily in Series A and Series B companies in software and software-enabled businesses serving healthcare and enterprise markets. The firm emerged as a spin-out from DFJ (Draper Fisher Jurvetson), retaining management of the existing DFJ portfolio while establishing its own brand and raising new funds. Threshold operates with a focused investment thesis, maintaining concentrated portfolios of high-conviction investments and providing active, hands-on partnership to founder-led companies during critical growth phases.

History

Threshold Ventures was founded in 2019 by Josh Stein and Emily Melton in Palo Alto, California, as a spin-out from the established venture capital firm DFJ (Draper Fisher Jurvetson). The firm retained management rights to DFJ's existing portfolio while launching under the Threshold brand with new capital raises. Since its founding, Threshold has established itself as a focused early-stage investor with offices in Palo Alto and New York, managing multiple funds including Threshold Ventures Select I (closed May 2022) and Threshold Ventures V (opened April 2025).

Product Lines

Product LinePositioningPrice Range
Series A InvestmentsEarly-stage software and healthcare technologyVaries by fund
Series B InvestmentsGrowth-stage software and enterprise solutionsVaries by fund
Healthcare Software FocusSoftware-enabled healthcare solutionsVaries by fund
Enterprise Software FocusBusiness software and SaaS platformsVaries by fund

Manufacturing

As a venture capital firm, Threshold Ventures does not manufacture physical products. The firm manages investment funds and provides capital and strategic guidance to portfolio companies. Threshold operates as an active investor with hands-on partnership, supporting founders throughout the investment cycle rather than producing goods or services directly.

Notable Products

  • Box - Cloud content management service backed by Threshold's investment team
  • Doximity - Digital platform for medical professionals
  • Twilio - Cloud communication platform
  • Redfin - Technology-based real estate brokerage
  • Rippling - HR platform for managing payroll and benefits
  • Remitly - Mobile payments service for international money transfers
  • Livongo - Company focused on empowering individuals with chronic conditions, later acquired by Teladoc

Reputation

Threshold Ventures is recognized as a disciplined, focused early-stage investor with deep expertise in healthcare and enterprise software markets. The firm is known for its hands-on partnership approach and long-term conviction in founder-led companies, with a track record of backing successful companies including Box, Twilio, and Doximity. The firm's concentrated portfolio strategy and emphasis on staying early, focused, and disciplined positions it as a deliberate partner rather than a high-volume investor, appealing to founders seeking active engagement during critical growth phases.

Sources (6)
  1. https://superscout.co/investor/threshold-ventures
  2. https://threshold.vc/about
  3. https://www.privateequityinternational.com/institution-profiles/threshold-ventures.html
  4. https://threshold.vc
  5. https://threshold.vc/companies
  6. https://adviserinfo.sec.gov/firm/summary/326413